DESCRIPTION: Long-read sequen cing technologies enable the sequencing of DNA fragments 10KB and longer. This read length greatly improves sequence mappability and assembly\, prov iding an advantage over short-read sequences that are difficult to map uni quely to repetitive and GC-rich regions. Long-read sequencing has applicat ions in a number of fields\, including genome assembly\, diagnosis of gene tic diseases\, and metagenomics. In this workshop\, we will focus on PacBi o HiFi sequences and introduce you to tools for haplotyping\, calling and visualizing structural variants and repeat expansions\, visualizing read m ethylation\, and detection of novel isoforms from PacBio Iso-Seq.
TE ACHERS: Madeline Couse (HPC4Health\, The Centre for Computational Medicine at SickKids) and Lauren Liang (HPC4Health\, Hospital for Sick Children)
LEVEL: Introductory
FORMAT: Lecture + Hands-on
CERTIFICAT E: Attendance
PREREQUISITE: Basic knowledge about DNA/RNA sequencing .
SUMMARY:Bioinformatics: Long-read Sequencing Applications URL;VALUE=URI:https://training.computeontario.ca/courses/course/view.php?id =30 END:VEVENT END:VCALENDAR