Biological research generates huge amounts of nex t-generation sequencing (NGS) data\, including RNA-seq. Scientists must ha ve specialized skills and knowledge to effectively collect\, analyze\, and interpret this data. While most bioinformatics tools require significant programming knowledge to use\, which presents a barrier to new bioinformat icians without a computational background\, the Galaxy platform allows res earchers to perform analyses using a graphical interface. Through lectur es and hands-on labs\, this 3-day French-language CBW course will provide participants with a comprehensive understanding of the fundamental princip les of RNA-seq analysis and the skills to perform this analysis themselves without needing to learn the command line. Each lecture-lab module is des igned as a distinct unit and all materials will be available open-access a fter the workshop concludes. Participants will work through a real case sc enario\, starting from a publication containing RNA-seq data\, moving to q uality control\, mapping\, gene quantification\, differential analysis\, a nd functional enrichment using gold standard tools (fastq-dump\, FASTQC\, fastp\, STAR\, featurecounts\, DESEQ2\, g:Profiler). We will also open a B ring your own data session\, giving the opportunity to talk with experts a bout their NGS projects.
SUMMARY:(2025) RNA-seq Using Galaxy en Français: Montréal\, QC URL;VALUE=URI:https://bioinformatics.ca/workshops-all/rna-seq-using-galaxy- en-francais/ END:VEVENT END:VCALENDAR